Targeting signal of the peroxisomal catalase in the methylotrophic yeast Hansenula polymorpha. |
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Authors: | T Didion R Roggenkamp |
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Institution: | Institut für Mikrobiologie, Heinrich-Heine-Universit?t Düsseldorf, Germany. |
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Abstract: | The methylotrophic yeast, Hansenula polymorpha, harbours a unique catalase (EC 1.11.1.6), which is essential for growth on methanol as a carbon source and is located in peroxisomes. Its corresponding gene has been cloned and the nucleotide sequence determined. The deduced amino acid sequence displayed the tripeptide serine-lysine-isoleucine at the extreme C-terminus, which is similar to sequences of other peroxisomal targeting signals. Exchange of the ultimate amino acid, isoleucine, of catalase for serine revealed a cytosolic enzyme activity and a concomitant loss of peroxisome function. We concluded that the tripeptide is essential for targeting of catalase in H. polymorpha. |
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