Immobilization of amyloglucosidase on polystyrene anion exchangeresin II. Kinetics and stabilities |
| |
Authors: | P. N. Nehete M. V. Hegde L. Gurucharan Reddy V. Shankar |
| |
Affiliation: | (1) Department of Chemistry, University of Poona, 411 007 Pune;(2) Division of Biochemical Sciences, National Chemical Laboratory, 411 008 Pune, India |
| |
Abstract: | Summary Amyloglucosidase (exo-1, 4- -D-glucosidase, EC 3.2.1.3), was coupled to glutaraldehyde activated Indion 48-R (a cross-linked macroporous anion exchanger) by Schiff base reaction. Immobilization brought about a marginal increase in the apparent Km. The bound enzyme exhibited increased stability towards urea and metal ions, but was less stable in the presence of guanidine hydrochloride. Immobilized amyloglucosidase could be stored at 4°C (in wet state) for 6–8 months without any apparent loss of activity. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|