Azo dye binding proteins as interfering factors in enzyme assays based on azo coupling |
| |
| Authors: | P L M?kinen K K M?kinen |
| |
| Affiliation: | 1. Department of Forensic Medicine, University of Turku, Turku 2, Finland;2. Institute of Dentistry, University of Turku, Turku 3, Finland;1. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina;2. Departamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. IMVIB-CONICET, Argentina;1. College of Pharmacy and Pharmaceutical Sciences, The University of Toledo Health Science Campus, Toledo, OH 43614, United States;2. Department of Bioengineering, The University of Toledo, Toledo, OH 43606, United States;3. Department of Surgery, The University of Toledo Health Science Campus, Toledo, OH 43614, United States;1. Department of Reconstructive Surgery of Osteo-articular Infections, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy;2. ASST Ovest Milanese, UOC Ortopedia e Traumatologia, Ospedale di Legnano, Milan, Italy;3. Hip Department, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy;4. Knee Department, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy;5. IRCCS Istituto Ortopedico Galeazzi, Laboratory of Clinical Chemistry and Microbiology, Milan, Italy;1. Department of Chemistry, University of Santiago de Chile, USACH, Chile;2. Center for Protein Study, Faculty of Biology, University of Havana, Havana, Cuba |
| |
| Abstract: | The azo coupling method used in the estimation of aminopeptidase and esterase activity is criticized. When N-l-aminoacyl-2-naphthylamines are used as substrates, certain proteins present in crude enzyme solutions as well as the amino acids liberated during the enzymic reaction severely interfere with the activity assay. Conditions are studied in which the azo dye binding caused by hemoglobin and albumin present in enzyme preparations produce the greatest interference. The effects described can lead to false fractionation patterns in column chromatography of amino-peptidases and esterases. The errors can be avoided when the azo dye is used in excessive amounts and if only small quantities of the proteins are present. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
