Differential biophysical properties of infectious intracellular and secreted hepatitis C virus particles |
| |
Authors: | Gastaminza Pablo Kapadia Sharookh B Chisari Francis V |
| |
Affiliation: | The Scripps Research Institute, Maildrop SBR-1010550, North Torrey Pines Road, La Jolla, CA 92037, USA. |
| |
Abstract: | The recent development of a cell culture infection model for hepatitis C virus (HCV) permits the production of infectious particles in vitro. In this report, we demonstrate that infectious particles are present both within the infected cells and in the supernatant. Kinetic analysis indicates that intracellular particles constitute precursors of the secreted infectious virus. Ultracentrifugation analyses indicate that intracellular infectious viral particles are similar in size (approximately 65 to 70 nm) but different in buoyant density (approximately 1.15 to 1.20 g/ml) from extracellular particles (approximately 1.03 to 1.16 g/ml). These results indicate that infectious HCV particles are assembled intracellularly and that their biochemical composition is altered during viral egress. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|