Development of multiple strain competitive index assays for Listeria monocytogenes using pIMC; a new site-specific integrative vector |
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| Authors: | Ian R Monk Pat G Casey Michael Cronin Cormac GM Gahan Colin Hill |
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| Affiliation: | (1) Department of Microbiology, University College Cork, Cork, Ireland;(2) Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland;(3) Department of Maths and Statistics, University College Cork, Cork, Ireland;(4) Department of Pharmacy, University College Cork, Cork, Ireland |
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| Abstract: | Background The foodborne, gram-positive pathogen, Listeria monocytogenes, is capable of causing lethal infections in compromised individuals. In the post genomic era of L. monocytogenes research, techniques are required to identify and validate genes involved in the pathogenicity and environmental biology of the organism. The aim here was to develop a widely applicable method to tag L. monocytogenes strains, with a particular emphasis on the development of multiple strain competitive index assays. |
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