A method for determination of 3,4-dihydroxyphenylacetic acid and 3,4-dihydroxymandelic acid in urine |
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| Authors: | B D Drujan N Alvarez J M Díaz Borges |
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| Affiliation: | 1. Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, 128 43 Prague 2, Czech Republic;2. J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Dolejškova 3, 182 23 Prague 8, Czech Republic |
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| Abstract: | A fluorometric method is described for the determination of urinary 3,4-dihydroxyphenylacetic acid (DOPAC) and 3,4-dihydroxymandelic acid (DOMA). This method is based upon ethyl acetate extraction, electrophoretic separation, and conversion of these compounds to highly fluorescent ethylenediamine condensation products.The normal urinary content of DOMA and DOPAC is given.The possibility of applying this method for the determination of dihydroxyphenylalanine (DOPA) is discussed. |
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