十三碳二元羧酸发酵技术的研究

以一株热带假丝酵母菌(Candida tropicalis) SP1为出发菌株，经紫外线反复诱变获取一株难以同化烷烃的突变株SPUV56，摇瓶培养5d平均产酸量达72g/L，较出发菌株提高了1.25倍，并利用突变株SPUV56在137L自控罐上扩试，补加醋酸盐发酵，144h产酸量达153g/L，比不加醋酸盐发酵提高了29.7%。采用提 高搅拌混合效果和低溶解氧发酵过程控制方法，可有效地提高菌体的产酸能力，在20m3发酵罐中发酵生产十三碳二元羧酸，总培养时间144h，产酸量可达172g/L,放罐体积15.0m3，产量为2.25t。

STUDY ON FERMENTATION OF 1,13-TRIDECANEDIOIC ACID BY CANDIDA TROPICALIS

A mutant of Candida tropicalis SP-1, SP-UV-56, which can produce 1,13-tride-canedioic acid or 1, 11-dicarboxylic acid (DCA13) 1.25 times as much as its parental strain SP-1, but hardly assimilates nalkane as carbon source for its growth, was obtained by ultraviolet treatments. A fermentation technology with a supplement of acetate at the latter middle of logarithmic growth was developed. Using sucrose as carbon source for its growth and supplying acetate the yield of DCA13 reached 153g/L for 144h incubation in a 13.7L autocontrolled stirred tank, about 29.7 % higher than that obtained in the broth without acetate.An effective technique that can maintain higher yield was provided by enhancing mixing of broth and controlling low DO level. With these techniques the DCA13 yield reached 172g/L in a 20m3 stirred tank, and produced 2.25 tons DCA13 in 15.0m3 broth.