Trs130 Participates in Autophagy Through GTPases Ypt31/32 in Saccharomyces cerevisiae |
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Authors: | Shenshen Zou Yong Chen Yutao Liu Nava Segev Sidney Yu Yan Liu Gaoyi Min Min Ye Yan Zeng Xiaoping Zhu Bing Hong Lars Olof Björn Yongheng Liang Shaoshan Li Zhiping Xie |
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Affiliation: | 1. College of Life Sciences, Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture, Nanjing Agricultural University, , Nanjing, 210095 China;2. Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, , Chicago, IL, 60607 USA;3. School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China;4. School of Medicine, Nankai University, , Tianjin, 300071 China;5. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, , Guangzhou, 510631 China |
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Abstract: | Trs130 is a specific component of the transport protein particle II complex, which functions as a guanine nucleotide exchange factor (GEF) for Rab GTPases Ypt31/32. Ypt31/32 is known to be involved in autophagy, although the precise mechanism has not been thoroughly studied. In this study, we investigated the potential involvement of Trs130 in autophagy and found that both the cytoplasm‐to‐vacuole targeting (Cvt) pathway and starvation‐induced autophagy were defective in a trs130ts (trs130 temperature‐sensitive) mutant. Mutant cells could not transport Atg8 and Atg9 to the pre‐autophagosomal structure/phagophore assembly site (PAS) properly, resulting in multiple Atg8 dots and Atg9 dots dispersed in the cytoplasm. Some dots were trapped in the trans‐Golgi. Genetic studies showed that the effect of the Trs130 mutation was downstream of Atg5 and upstream of Atg1, Atg13, Atg9 and Atg14 on the autophagic pathway. Furthermore, overexpression of Ypt31 or Ypt32, but not of Ypt1, rescued autophagy defects in trs130ts and trs65ts (Trs130‐HA Trs120‐myc trs65Δ) mutants. Our data provide mechanistic insight into how Trs130 participates in autophagy and suggest that vesicular trafficking regulated by GTPases/GEFs is important in the transport of autophagy proteins from the trans‐Golgi to the PAS. |
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Keywords: | autophagy GEF complex Rab GTPases TRAPP II Ypt31/32 |
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