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Characterization of the DNA of Hyalophora cecropia
Authors:Daniel D. Burke  David Rubin  Judith H. Willis
Affiliation:Departments of Microbiology and Entomology, University of Illinois, Urbana, Illinois 61801, U.S.A.
Abstract:DNA was isolated from the pupae and various tissues of pharate adults of the silkmoth Hyalophora cecropia. CsCl equilibrium density analysis showed the presence of one major band at 1.693 and two minor satellites, 1.705 and 1.709, comprising 3 and 6% respectively of the total DNA. We could detect no difference in the renaturation kinetics of DNA prepared from pupal or pharate adult tissues. The genome appears to be composed of 30% redundant and 70% unique sequences. The haploid information content of the unique sequences is 11.4 × 1010 daltons.
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