Co-fermentation of cassava waste pulp hydrolysate with molasses to ethanol for economic optimization |
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Authors: | Thippawan Wattanagonniyom Wen-Chien Lee Vasana Tolieng Somboon Tanasupawat Ancharida Akaracharanya |
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Institution: | 1.Department of Microbiology, Faculty of Science,Chulalongkorn University,Pathumwan,Thailand;2.Department of Chemical Engineering,National Chung Cheng University,Minhsiung,Taiwan;3.Institute of Biotechnology and Genetic Engineering,Chulalongkorn University,Pathumwan,Thailand;4.Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Science,Chulalongkorn University,Pathumwan,Thailand |
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Abstract: | Cassava waste pulp (CWP)–enzymatic hydrolysate was co-fermented with molasses (CWP-EH/molasses mixture) with the aim to optimize ethanol production by Saccharomyces cerevisiae TISTR 5606 (SC 90). The optimal fermentation conditions for ethanol production using this mixture were 245 g/L initial total sugar supplemented with KH2PO4 (8 g/L), at 30 °C for 48 h of fermentation under an oxygen-limited condition with agitation at 100 rpm, producing an ethanol concentration of 70.60 g/L (0.31 g ethanol/g total sugar). The addition of cassava tuber fiber (solid residue of CWP after enzymatic hydrolysis) at 30 g/L dry weight to the CWP-EH/molasses mixture increased ethanol production to 74.36 g/L (0.32 g ethanol/g total sugar). Co-fermentation of CWP-EH with molasses had the advantage of not requiring any supplementation of the fermentation mixture with reduced nitrogen. |
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