The hybridization of DNA from two species of honeybee |
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| Authors: | Andrew Sperlin Roy Campbell Ronald W. Brosemer |
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| Affiliation: | 1. Program in Biochemistry and Biophysics, Washington State University, Pullman, Washington 99163, U.S.A.;2. Department of Chemistry, Washington State University, Pullman, Washington 99163, U.S.A. |
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| Abstract: | DNA was isolated from the Indian honeybee (Apis cerana) and from the Italian and Anatolian races of the true honeybee (A. mellifera). The single-copy fraction of each DNA was labelled with iodine-125. Labelled single-stranded single-copy DNA from one species was allowed to associate with excess unlabelled single-stranded DNA from the other species. Hydroxylapatite chromatographic analysis of the heterohybrids showed that about 60% of the genomes are similar enough to form hybrids with a high degree of base pairing. About 40% of the genomes are so dissimilar that they cannot form hybrids stable under the experimental conditions of reassociation. |
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