Conjugational delivery of chromosomal integrative constructs for gene expression in the carbendazim-degrading <Emphasis Type="Italic">Rhodococcus erythropolis</Emphasis> D-1 |
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Authors: | Yang Tao Wen-Guang Wang Sheng-Hui Lu Xin Xie Ugit Lazzat Naling Bai Yuhua Zhao Hui Jiang |
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Institution: | 1.College of Life Sciences,Zhejiang University,Hangzhou,China;2.Eco-environmental Protection Institute,Shanghai Academy of Agricultural Sciences,Shanghai,China |
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Abstract: | Rhodococcus strains not only have been widely used in industries but also have a potential ability of producing new structural natural products. Integration of heterologous genes into chromosomes of Rhodococcus strains for gene expression can facilitate the studies and applications of these strains. A conjugation system was optimized in order to transfer enhanced green fluorescent protein (EGFP) encoding gene as a reporter from Escherichia coli into Rhodococcus erythropolis D-1. The influence of three native ribosome binding sites (RBSs) and two designed RBSs on the target protein production in R. erythropolis D-1 was also characterized. An efficient conjugation system of R. erythropolis D-1 was established to integrate EGFP gene into its chromosome. Among of five RBSs, RBS3 showed the highest translational activity in R. erythropolis D-1. |
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