Next‐generation phylogenetics takes root |
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Authors: | John E McCormack Brant C Faircloth |
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Institution: | 1. Moore Laboratory of Zoology, Occidental College, , Los Angeles, CA, 90041 USA;2. Department of Ecology & Evolutionary Biology, University of California, , Los Angeles, CA, 90095 USA |
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Abstract: | It has been a tumultuous 5 years in phylogeography and phylogenetics during which both fields have struggled to harness the power of next‐generation sequencing (NGS) (Ekblom & Galindo 2010 ; McCormack et al. 2012a ). Fortunately, several methodological approaches appear to be taking root. In this issue of Molecular Ecology, O'Neill et al. 2013 ) employ one such method – parallel tagged sequencing (PTS) – to elucidate the phylogeography of a tiger salamander (Ambystoma tigrinum) species complex. This study demonstrates a practical application of NGS on a scale appropriate (and not overkill) for most biologists interested in phylogeography (~100 loci for ~100 individuals), and their results highlight several analytical challenges that lie ahead for researchers employing NGS techniques. |
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