The metabolic integrity of Fasciola hepatica during in vitro maintenance |
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Authors: | Roslyn A. Cornish C. Bryant |
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Affiliation: | Department of Zoology, Australian National University, A.C.T., Australia |
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Abstract: | Levels of metabolic intermediates and end products in F. hepatica after 24 and 48 h in Hédon-Fleig salt solution with added glucose were compared with levels obtained immediately on removal from the host. Glycogen levels dropped initially, probably due to the expulsion of eggs; thereafter they remained constant. Internal glucose concentrations increased as the parasites equilibrated with the medium. Other changes in internal pool sizes were consistent with regulation to the in vitro conditions. ATP levels increased; ATP/ADP ratios were maintained. Comparisons of mass action ratios and equilibrium constants suggest that hexokinase, pyruvate kinase and phosphofructokinase are regulatory. Output of excretory products approached linearity; from the calculated regressions the proportions of lactate, acetate and propionate were 1: 2: 4. The implications for metabolic regulation in F. hepatica are briefly discussed, and it is concluded that, for at least 48 h in vitro, energy metabolism is not adversely affected. |
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Keywords: | metabolic intermediates end products adenine nucleotides ATP Adenine triphosphate ADP Adenine diphosphate AMP Adenine monophosphate F6P fructose-6-phosphate FDP fructose-1, 6-diphosphate G6P glucose-6-phosphate NAD(H) nicotinamide adenine dinucleotide (reduced) PEP phosphoenolpyruvate 2PGA 2-phosphoglycerate 3PGA 3-phosphoglycerate TP triose phosphates |
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