金属离子对黑米花青苷色素吸收光谱的影响

以黑糯B糙米皮为实验材料 ,用 1 .5mol/L盐酸— 95 %乙醇 (V/V :1 5 / 85 )溶液提取黑米花青苷色素(BRAP) ,采用紫外可见分光光度法研究了 1 1种金属离子以及 (NH4 ) 1+ 离子对BRAP的作用。结果表明 ,未加离子条件下色素溶液可见光区λmax5 35nm ,紫外光区λmax2 80nm ,加入Al3 + 、Fe3 + 、Fe2 + 、Cu2 + 、Mn2 + 、Zn2 + 、Sn2 + 对其吸收光谱有显性影响。其中Al3 + 、Fe3 + 使 5 35nm特征吸收峰发生蓝移 ,Sn2 + 使其发生明显红移 ;Al3 + ,Fe2 + ,Mn2 + ,Zn2 +在 5 35nm附近有增加ABS值作用 ,Fe3 + 有减小ABS值作用 ;延长作用时间 ,Cu2 + 对BRAP吸收光谱的影响表现为λmax5 35nm发生蓝移 ,ABS值减小

Influence of Metal Ions on the Absorption Spectrum of Black Rice Anthocyanin Pigment

The effect of 11 metal ions and (NH 4) 1+ on black rice anthocyanin pigment(BRAP) was investigated by UV-VIS spectrometry. The pigment solution without metal ions showed the λmax535nm in VIS-region and λmax280nm in UV-region. When Al 3+ ,Fe 3+ ,Fe 2+ ,Cu 2+ ,Mn 2+ ,Zn 2+ and Sn 2+ added separately, the solution was obviously influenced. Among them, the Al 3+ and Fe 3+ made the characteristic absorption peak move to blue λmax 535nm and the Sn 2+ made it red shift. When Al 3+ ,Fe 2+ ,Mn 2+ ,Mn 2+ and Zn 2+ were added in the pigment solution respectively, the ABS value near 535nm was increased, but Fe 3+ made it reduce. Extending the time of Cu 2+ reaction, the pigment solution λ max 535nm shift to blue and the ABS value reduced.