Auxin-activated nadh oxidase activity of soybean plasma membranes is distinct from the constitutive plasma membrane NADH oxidase and exhibits prion-like properties |
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Authors: | Email author" target="_blank">D?James?MorréEmail author Dorothy?M?Morré Philipp?Ternes |
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Institution: | (1) Department of Medicinal Chemistry, Purdue University, 47907 West Lafayette, IN;(2) Foods and Nutrition Department, Purdue University, 47907 West Lafayette, IN;(3) Institut für Allgemeine Botanik, University of Hamburg, Hamburg, Germany |
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Abstract: | Summary The hormone-stimulated and growth-related cell surface hydroquinone (NADH) oxidase activity of etiolated hypocotyls of soybeans
oscillates with a period of about 24 min or 60 times per 24-h day. Plasma membranes of soybean hypocotyls contain two such
NADH oxidase activities that have been resolved by purification on concanavalin A columns. One in the apparent molecular weight
range of 14–17 kDa is stimulated by the auxin herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The other is larger and unaffected
by 2,4-D. The 2,4-D-stimulated activity absolutely requires 2,4-D for activity and exhibits a period length of about 24 min.
Also exhibiting 24-min oscillations is the rate of cell enlargement induced by the addition of 2,4-D or the natural auxin
indole-3-acetic acid (IAA). Immediately following 2,4-D or IAA addition, a very complex pattern of oscillations is frequently
observed. However, after several hours a dominant 24-min period emerges at the expense of the constitutive activity. A recruitment
process analogous to that exhibited by prions is postulated to explain this behavior. |
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Keywords: | NADH oxidase ECTO-NOX proteins growth auxin prion |
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