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A method for the determination of the integrity of labeled GABA used in membrane receptor binding assay
Authors:A Cupello  G Besio  P Mainardi
Institution:(1) Centro di Neurofisiologia Cerebrale, C.N.R. and Clinica Neurologica, Università di Genova, via De Toni 5, 16132 Genova, Italy
Abstract:A simple method for the determination of the proportion of true GABA within labeled ldquoGABArdquo used for membrane binding assay is presented. The method is intended for the assessment of the integrity of refrigerator (+4°C) stored labeled neurotransmitter. Its application allows a precise determination of the binding parameters.
Keywords:GABA  GABA breakdown  receptor binding
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