Enhancement of oxygen transfer in Hybridoma cell culture by using a perfluorocarbon as an oxygen carrier |
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Authors: | Moo H. Cho Shaw S. Wang |
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Affiliation: | (1) Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, PO BOX 909, 08854 Piscataway, NJ, USA;(2) Present address: Department of Chemical Engineering, Yeungnam University, Gyongean, Korea |
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Abstract: | Summary In order to increase the oxygen transfer in a bioreactor for Hybridoma cell culture, a perfluorocarbon, FlutecR ppll was used in a modified CelligenTM system. There was no harmful effect of ppll on the cell growth and on the production of monoclonal antibody. |
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