Enzyme-linked immunosorbent assay for Aeromonas hydrophila hemolysins |
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| Authors: | Shunji Kozaki Akane Kurokawa Tsutomu Asao Keiko Kato Takashi Uemura Genji Sakaguchi |
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| Affiliation: | Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Osaka, Japan;Osaka Prefectural Institute of Public Health, Osaka, Japan |
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| Abstract: | The enzyme-linked immunosorbent assay (ELISA) was applied to determine hemolysins produced by Aeromonas hydrophila. This assay detected quantitatively each of the 2 immunologically distinct hemolysins with its homologous anti-hemolysin. The hemolysins produced by 40 isolates from different sources were separable into 2 types, with identical or at least similar antigenicity to either of the 2 hemolysins. Cholera-like enterotoxin was not detected in any strain by GM1-ELISA. The ELISA systems may be valuable in clarifying the role of hemolysin in A. hydrophila diarrheal disease. |
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| Keywords: | Aeromonas hydrophila Haemolysin ELISA |
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