Camptothecin binds to a synthetic peptide identified by a T7 phage display screen |
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Authors: | Takakusagi Yoichi Kobayashi Susumu Sugawara Fumio |
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Affiliation: | Genome and Drug Discovery Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. |
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Abstract: | An analysis of non-biotinylated camptothecin (CPT) binding to the C-20-biotinylated CPT binding peptide NSSQSARR was carried out using two methods, quartz-crystal microbalance (QCM) and surface plasmon resonance (SPR). The peptide was immobilized peptide on a sensor chip and showed a dissociation constant (KD) of approximately 0.1 microM against CPT in QCM and SPR experiments. |
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