Changes in cotyledon mRNA during floral induction of Pharbitis nil CV. Violet |
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Authors: | M. Lay-Yee R. M. Sachs M. S. Reid |
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Affiliation: | (1) Department of Environmental Horticulture, University of California, 95616 Davis, CA, USA;(2) Present address: Division of Horticulture and Processing, Department of Scientific and Industrial Research, DSIR, Private Bag, Auckland, New Zealand |
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Abstract: | Floral induction in seedlings of Pharbitis nil Choisy cv. Violet, with one cotyledon removed, was manipulated by applying various photoperiodic treatments to the remaining cotyledon. Populations of polyadenylated RNA from treated cotyledons were examined to identify messages specifically involved in floral induction. The RNA was translated in vitro using a wheat-germ system, and the resulting translation products were analysed by two-dimensional polyacrylamide gel electrophoresis. Substantial qualitative and quantitative differences were found between mRNA from cotyledons of seedlings kept in continuous light (non-induced) and of seedlings given a 16-h dark period (induced). In contrast, inhibition of flowering with a night-break resulted only in one detectable, quantitative difference in mRNA.Abbreviations CL continuous light - kDa kilodalton - NB 16 h darkness+10 min red-light break, 8 h into the dark period - poly(A)+ RNA polyadenylated RNA (isolated by binding to a cellulose oligodeoxythymidine affinity column) - SD short day (16 h dark) - SDP short-day plant - SDS sodium dodecyl sulfate |
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Keywords: | Floral induction (gene expression) mRNA and floral induction Pharbitis Photoperiodism (flowering) Short-day plant |
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