Schistosoma mansoni: nucleic acid synthesis in immature females from single-sex infections, paired in vitro with intact males and male segments |
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Authors: | P F Basch |
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Institution: | Department of Family, Community and Preventive Medicine, Stanford University School of Medicine, CA 94305. |
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Abstract: | 1. The object of this study was to see whether stimulation of nucleic acid synthesis in immature females by male Schistosoma mansoni is mediated locally by contact, or is propagated systemically in the female. 2. Immature females perfused from single-sex animal infections were paired for one week in vitro with segments of males cut transversely into thirds; others were paired with intact males, or maintained without males; all were then incubated with 3H]-thymidine or tyrosine. 3. Washed females were bisected transversely and isotope uptake counted separately in the anterior and posterior halves. 4. The halves in contact with cut male segments showed significantly higher uptake of 3H]-thymidine than the non-contact halves, indicating increased DNA synthesis and cell division, but non-contact halves had greater uptake of 3H]-tyrosine. 5. Dot-blot hybridization with a female specific single stranded cDNA failed to detect production of the corresponding mRNA in females paired with male segments. |
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