Ultrastructure of infected cells in the actinorhizal root nodules ofGymnostoma papuanum (Casuarinaceae) prepared by high pressure freezing and chemical fixation |
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| Authors: | W. Newcomb S. Jackson S. Racette J. G. Torrey |
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| Affiliation: | (1) Department of Biology, Queen's Univerity, K7L 3N Kingston, Ont., Canada;(2) Present address: Department of Botany, York University, North York, Ontario, Canada;(3) Harvard Forest, Harvard University, Petersham, Massachusetts |
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| Abstract: | Summary Actinorhizal root nodules ofGymnostoma papuanuum (Casuarinaceae) were examined with transmission electron microscopy after being either fixed with glutalaldehyde and osmium tetroxide or frozen with liquid nitrogen at high pressure and freeze-substituted. Much better preservation was obtained by the cryopreservation method. Mitochondria, plastids, membranes und ribosomes were much better preserved in the frozen specimens than in the chemically fixed tissues. No nucleoids were observed in the microsymbiont in frozen specimens. In contrast nucleoid regions were present in chemically fixed specimens. The actinomycete microsymbiont differentiates small spherical-shaped symbiotic vesicles after the hyphae have grown and penetrated into most regions of the cytoplasm.Dedicated to the memory of Professor Oswald Kiermayer |
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| Keywords: | Cryopreservation High pressure freezing Actinomycete Nitrogen-fixation |
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