n-Alkane dissimilation by Rhodopseudomonas sphaeroides transferred OCT plasmid |
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Authors: | M -H Lee M -O Hwang S -Y Choi K -H Min |
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Institution: | (1) Department of Biology, Sookmyung Women's University, 140-742 Seoul;(2) Research Center for Molecular Microbiology, Seoul National University, 151-742 Seoul, Korea |
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Abstract: | The OCT plasmid from Pseudomonas maltophilia N246-1 was transferred to Rhodopseudomonas sphaeroides M1 with very low frequency (1.4–1.9 × 10–5 per recipient cell at pH 7–8 for a 3-hour reaction time). P. maltophilia N246-1 was able to utilize C8-C14 of n-alkanes, whereas R. gas-liquid chromatography determined that the broad range of carbon numbers of n-alkanes in crude oil was remarkably degraded by the transconjugant, R. sphaeroides M1-C1, compared with donor strain N246-1. The fact that donor and transconjugant strains simultaneously lost the capacity to utilize n-alkanes on L-broth medium suggests that the OCT plasmids are unstable. It was found that the OCT plasmid of P. maltophilia N246 was incompatible with the IncP-2 group of P. aeruginosa KCTC 11245.
Offprint requests to: K.-H. Min, Sookmyung Women's University. |
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