Purification of polymeric phospholipase Cs from human platelets |
| |
| Authors: | T Moriyama H Narita M Oki T Matsuura M Kito |
| |
| Affiliation: | Research Institute for Food Science, Kyoto University. |
| |
| Abstract: | Two types of cytosolic phospholipase C specific for phosphoinositides were purified from human platelets. The molecular masses of the purified enzymes were 440 and 290 kDa. These enzymes were concluded to be respectively a trimer and a dimer of homologous 146 kDa polypeptides. The 146 kDa polypeptide may be an immunologically novel isozyme among the 140-150 kDa PLC isozymes. Both enzymes hydrolyzed phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate in a Ca2(+)-dependent manner. |
| |
| Keywords: | |
|
