Detection of protein Z in a renal calculus composed of calcium oxalate monohydrate with the use of liquid chromatography-mass spectrometry/mass spectrometry following two-dimensional polyacrylamide gel electrophoresis separation |
| |
Authors: | Kaneko Kiyoko Yamanobe Tomoyo Nakagomi Kazuya Mawatari Ken-ichi Onoda Maki Fujimori Shin |
| |
Institution: | Central Laboratory of Analytical Biochemistry, Teikyo University, Tokyo 173-8605, Japan. kikaneko@pharm.teikyo-u.ac.jp |
| |
Abstract: | Protein Z, a vitamin K-dependent plasma protein, has been detected for the first time in a renal calculus along with osteopontin and prothrombin. The renal calculus was obtained from a hyperuricemic patient. Following two-dimensional polyacrylamide gel electrophoresis, the calculus was analyzed with the use of liquid chromatography mass spectrometry (LC-MS). The spectrometer was equipped with a nanoelectrospray interface and an ion trap. Four peptides were determined from a protein in the calculus through LC-MS/MS analysis. Tandem mass spectrum database matching tools were used to identify the protein as protein Z. Authentic protein Z was also analyzed using the same method, and all four peptides determined in the calculus were similarly identified. Whereas protein Z has been reported to be one of the vitamin K-dependent calcium-binding proteins, its role has not been well established. The fact that protein Z exists in a renal calculus composed of calcium oxalate will be beneficial in any future investigations into its role in the body. |
| |
Keywords: | Protein Z Renal calculus 2D-PAGE separation LC-MS/MS Micro-area X-ray diffractometer Calcium oxalate monohydrate |
本文献已被 ScienceDirect PubMed 等数据库收录! |