A monoclonal antibody that recognizes both leb and Y (Ley) antigens |
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| Authors: | Thomas Brodin M Alan Chester Karl-Anders Karlsson Lisbeth Messeter David Zopf Arne Lundblad |
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| Institution: | (1) Department of Tumor Immunology, Wallenberg Laboratory, University of Lund, Box 7031, S-220 07 Lund, Sweden;(2) BioCarb AB, S-223 70 Lund, Sweden;(3) Department of Biochemistry, University of Gothenburg, PO Box 33031, S-400 33 Gothenburg, Sweden;(4) Blood Centre, University of Lund, S-221 85 Lund, Sweden;(5) National Cancer Institute, National Institutes of Health, 20892 Bethesda, MD, USA;(6) Department of Clinical Chemistry, University of Lund, S-221 85 Lund, Sweden |
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| Abstract: | A hemagglutinating monoclonal antibody has been obtained from a mouse/mouse hybridoma after immunisation with the leb-active oligosaccharide, lacto-N-difucohexaose I, coupled to edestin. The antibody agglutinated human red cells regardless of Lewis phenotype. Blood group O cells were strongly, agglutinated, and progressively weaker agglutination was observed with A2, B and A2B cells. Blood group A1 and A1B cells were not agglutinated.By examining the binding of the antibody to glycolipids and oligosaccharides it was shown that the Leb and Y (Ley)-haptens bind to a similar extent. Full binding activity was dependent on the presence of, both fucosyl residues.Abbreviations LND l
lacto-N-difucohexaose l
- IV2Fuc,lll4FucLcOse4
LND l-OL, lacto-N-difucohexaitol l |
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| Keywords: | monoclonal antibody blood groups carbohydrate specificity |
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