Genetic control of flocculation inEscherichia coli |
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| Authors: | Kimberly L Ogden Austin L Taylor |
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| Institution: | (1) Department of Chemical Engineering, University of Colorado, 80309-0424 Boulder, Colorado, USA;(2) Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver, Colorado, USA |
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| Abstract: | Summary
Escherichia coli cells form flocs or aggregates by overproducing type 1 pili. When thepil operon is placed under the control of atac orlac promoter-operator sequence, the bacterial cells can be induced to form flocs by adding isopropyl- -d-thiogalactopyranoside to the culture medium. This phenomenon of genetically induced flocculation can aid in the downstream processing of biological products. This paper describes the construction of two artificially controlled plasmids which cause cell flocculation. Cell aggregates 50  m in mean diameter were obtained 1 h after the cells were induced. |
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| Keywords: | Bacterial aggregation Induction Type 1 pili |
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