产木聚糖酶芽胞菌Y-12的筛选及产酶条件的研究

利用透明圈平板培养和木聚糖发酵试验,筛选出1株高产木聚糖酶的芽胞杆菌,并对该菌株的酶学特性和适宜发酵条件进行研究。结果表明,此菌对秸秆和麦麸的分解率分别为43.3%和63.4%。产酶较高的最适培养条件,即大豆蛋白胨2%、胰蛋白胨3%、酵母粉0.1%和葡萄糖2%。适宜反应温度和pH分别为37℃和7。采用10 L发酵罐发酵,培养48 h后,酶活达3024 U/m l,比三角瓶发酵(酶活达2185 U/m l)提高了38%。

Screening of xylanase producing Bacillus and study on its enzyme production conditions

A high-xylanase-producing Bacillus strain was screened by transparent zone and xylan assay on the selective medium.Its proper conditions for fermentation and reaction were investigated.Results indicated that the strains could degrade straw and wheat bran,with the degradation rates at 43.3% and 63.4% respectively.On the basis of screening for liquid culture and determination of activity,an optimal culture conditions with higher yield was obtained,which contained 2% of soybean meal,3% of tryptone,0.1% of yeas...