Negative mode nanostructure-initiator mass spectrometry for detection of phosphorylated metabolites |
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Authors: | Andrea Amantonico Luca Flamigni Reto Glaus Renato Zenobi |
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Institution: | (1) Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland; |
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Abstract: | The chemical complexity of the metabolome requires the development of new detection methods to enlarge the range of compounds
detectable in a biological sample. Recently, a novel matrix-free laser desorption/ionization method called nanostructure-initiator
mass spectrometry (NIMS) Northen et al., Nature 449(7165):1033–1036, 2007] was reported. Here we investigate NIMS in negative
ion mode for the detection of endogenous metabolites, namely small phosphorylated molecules. 3-Aminopropyldimethylethoxysilane
was found to be suitable as initiator for the analytes studied and a limit of detection in the tens of femtomoles was reached.
The detection of different endogenous cell metabolites in a yeast cell extract is demonstrated. |
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Keywords: | |
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