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Polymorphic microsatellite loci for studies of the Ozark hellbender (Cryptobranchus alleganiensis bishopi) |
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| Authors: | Jarrett R Johnson Kaitlyn M Faries Jessica J Rabenold Rachel S Crowhurst Jeffrey T Briggler Jeffrey B Koppelman Lori S Eggert |
| Institution: | 1. Division of Biological Sciences, University of Missouri, 226 Tucker Hall, Columbia, MO, 65211, USA 3. Section of Evolution and Ecology, University of California, Davis, CA, 95616, USA 2. Missouri Department of Conservation, Resource Science Center, Columbia, MO, 65201, USA |
| Abstract: | The hellbender is the only North American member of the aquatic salamander family Cryptobranchidae and is a species of conservation concern across its range. We developed eight polymorphic microsatellite loci for hellbenders using a magnetic bead enrichment protocol and a PCR-based detection technique. Allelic diversity averaged 4.0 (±1.8 SD) per locus and heterozygosity averaged 0.56 (±0.30 SD). The hellbender is rare and difficult to study due to its cryptic life history. These loci will provide a valuable resource for population studies, which could inform future conservation and management decisions. |
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