Analyses oftraA, int-Tn,andxis-TnMutations in the Conjugative Transposon Tn916inEnterococcus faecalis |
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| Authors: | Deborah D. Jaworski Susan E. Flannagan Don B. Clewell |
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| Affiliation: | aDepartment of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan, 48109;bDepartment of Microbiology and Immunology, School of Medicine, University of Michigan, Ann Arbor, Michigan, 48109 |
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| Abstract: | The conjugative transposon Tn916moves intercellularly via an excision/insertion mechanism that involves products ofint-Tnandxis-Tn.Tn5-insertion mutations in these genes were found to be complemented in anEnterococcus faecalishost by specific coresident transposons harboring the corresponding wild-type allele. A determinant designatedtraA,partially overlapping and divergently transcribed fromxis-Tn,is thought to encode a key positively acting regulatory protein needed for expression of conjugation functions. This locus was also shown to express atrans-acting product. |
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