Autoradiographic Evidence of [3H]SCH 23390 Binding Site; in Human Prefrontal Cortex (Brodmann's Area 9) |
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| Authors: | Ted M Dawson R Tyler McCabe Suzanne S Stensaas James K Wamsley |
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| Institution: | Department of Psychiatry and Pharmacology, University of Utah School of Medicine, Salt Lake City, Utah, U.S.A.;Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, U.S.A. |
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| Abstract: | The distribution of dopamine D-1 receptors has been determined in human prefrontal cortex (Brodmann's area 9) by an in vitro light microscopic autoradiographic method. Dopamine D-1 receptors were localized by using 3H]SCH 23390 as a ligand. Our results indicated that 3H]SCH 23390 binding to slide-mounted tissue sections of human brain is specific, saturable, and of high affinity. Lamina Va contained the highest density of D-1 receptors, with a Bmax value of 11.2 +/- 1.3 fmol/mg tissue. The KD values for 3H]SCH 23390 in all laminae ranged from 2.6 to 3.2 nM. Competition studies performed with 3H]SCH 23390 indicated a pharmacologic profile consistent with labeling of the D-1 receptor. |
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| Keywords: | Human brain Prefrontal cortex Brodmann's area 9 [3H]SCH 23390 Dopamine D-l receptor Autoradiography |
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