Preparation of conophylline affinity nano-beads and identification of a target protein |
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| Authors: | Eriko Suzuki Hideki Ogura Kuniki Kato Izumi Takei Yasuaki Kabe Hiroshi Handa Kazuo Umezawa |
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| Affiliation: | aDepartment of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-0061, Japan;bTokyo Dental College Ichikawa General Hospital, 5-11-13 Kanno, Ichikawa 272-8513, Japan;cGraduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku, Yokohama 226-8503, Japan |
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| Abstract: | Conophylline, a vinca alkaloid extracted from the tropical plant Ervatamia microphylla, has been shown to induce the differentiation of insulin-producing β-cells in cultured cells and in animals. However, its mechanism of action and the molecular target have remained unclear. Therefore, we prepared a fishing probe with conophylline to identify the target protein by using latex nano-beads, which are newly innovated tools for affinity-purification. With these conophylline-linked nano-beads, we found that conophylline directly interacted with ARL6IP. ARL6IP may thus be involved in the mechanism of cellular differentiation of β-cells, and this probe should be useful to find other target proteins. |
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| Keywords: | Conophylline Amination Nano-beads Affinity-purification ARL6IP |
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