RNA interference mediated knockdown of apolipophorin-III leads to knockdown of manganese superoxide dismutase in Hyphantria cunea |
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| Authors: | Kim Yong Il Kim Hong Ja Kwon Yong Min Kang Young Jin Lee In Hee Jin Byung Rae Han Yeon Soo Kim Iksoo Cheon Hyang Mi Ha Nam Gyu Seo Sook Jae |
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| Institution: | a Division of Applied Life Science, Gyeongsang National University, Jinju, Republic of Korea;b Department of Pharmacology, Yeungnam University, Daegu, Republic of Korea;c Department of Life Science, Hoseo University, Asan, Republic of Korea;d College of Natural Resources and Life Science, Dong-A University, Busan, Republic of Korea;e Department of Agricultural and Life Sciences, Chonnam National University, Gwangju, Republic of Korea;f Southern Forest Research Center, Korea Forest Research Institute, Jinju, Republic of Korea;g Agricultural Experiment and Research Bureau, Gyeongnam, Agricultural Research and Extension Services, Jinju, Republic of Korea |
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| Abstract: | Apolipophorin-III (apoLp-III), a hemolymph protein that facilitates lipid transport in aqueous media in insects was recently shown to play a role in insect immune activation. Here, we report another novel possible function of apoLp-III in insects. To identify genes affected by apoLp-III expression in larvae, we decreased endogenous apoLp-III mRNA in Hyphantria cunea (Hc) through RNA interference; subsequently, we observed lower levels of antioxidant enzymes, including manganese superoxide dismutase (MnSOD), glutathione S-transferase, and immune proteins. Knockdown of Hc apoLp-III led to decreased MnSOD expression in fat body tissues and elevated superoxide anion levels in Hc fat body cells, suggesting that Hc apoLp-III is involved in the action and/or expression of antioxidant enzymes, especially MnSOD. |
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| Keywords: | Apolipophorin-III RNAi Manganese superoxide dismutase Antioxidant enzyme Reactive oxygen species |
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