Reconstitution of a Ca2+-transporting ATPase system from triton X-100-solubilized sarcoplasmic reticulum. |
| |
| Authors: | M Chiesi S W Peterson O Acuto |
| |
| Institution: | Laboratory of Biochemistry, Swiss Federal Institute of Technology (ETH), 8092 Zürich, Switzerland |
| |
| Abstract: | Ca2+ ATPase activity and Ca2+ transport from Triton X-100-solubilized sarcoplasmic reticulum vesicles and soybean phospholipids were reconstituted by passing this mixture through a Bio-Bead SM-2 column. This rapid procedure gave a coupling efficiency of 0.83 mol of Ca2+-mol? of ATP hydrolyzed when 35 mg of soybean phospholipids mg?1 of protein was used. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
