A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR |
| |
Authors: | Hellein Kristen N Kennedy Elizabeth M Harwood Valerie J Gordon Katrina V Wang Shiao Y Lepo Joe Eugene |
| |
Affiliation: | Center for Environmental Diagnostics and Bioremediation, University of West Florida, 11000 University Parkway, Pensacola, FL 32514, USA. |
| |
Abstract: | Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|