A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR |
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| Authors: | Hellein Kristen N Kennedy Elizabeth M Harwood Valerie J Gordon Katrina V Wang Shiao Y Lepo Joe Eugene |
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| Institution: | Center for Environmental Diagnostics and Bioremediation, University of West Florida, 11000 University Parkway, Pensacola, FL 32514, USA. |
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| Abstract: | Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings. |
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