Anthraquinones as phytoalexins in cell and tissue cultures of Cinchona spec. |
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| Authors: | R Wijnsma J T K A Go I N van Weerden P A A Harkes R Verpoorte A Baerheim Svendsen |
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| Institution: | (1) Center for Bio-Pharmaceutical Sciences, Division of Pharmacognosy, State University of Leiden, Gorlaeus Laboratories, P.O. Box 9502, NL-2300 RA Leiden, The Netherlands;(2) Department of Molecular Botany, Subfaculty of Biology, State University of Leiden, The Netherlands |
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| Abstract: | The addition of autoclaved mycelia of Aspergillus niger and the known phytopathogenic fungus Phytophtora cinnamomi to cultured cells of Cinchona ledgeriana Moens. caused a marked increase in the anthraquinone content of the plant cells. This finding in combination with the antimicrobial activity of the anthraquinones isolated from calli of Cinchona pubescens Vahl. led to the conclusion that anthraquinones are phytoalexins.Abbreviations 2,4-D
2,4-dichlorophenoyacetic acid
- TLC
thin-layer chromatography
- AQ
anthraquinone
- DW
dry weight |
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