Folding kinetics of mammalian ribonucleases |
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Authors: | K Lang A Wrba H Krebs F X Schmid J J Beintema |
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Affiliation: | 1. Institut für Biophysik und Physikalische Biochemie, Universität Regensburg, D-8400 Regensburg, FRG;2. Biochemisch Laboratorium, Rijksuniversiteit Groningen, Nijenborgh 16, NL-9747 AG Groningen, The Netherlands |
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Abstract: | The folding kinetics of seven different pancreatic ribonucleases are compared both under native conditions and within the unfolding transition. In general, the folding kinetics of these proteins are similar despite numerous amino acid substitutions. Ribonucleases with 4-6 proline residues show 80% slow-folding species. For three ribonucleases with 7 prolines this number increases to 90%. Porcine ribonuclease with a unique Pro 114-Pro 115 sequence folds significantly slower than other ribonucleases which do not show this sequence. |
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