Rapid plant regeneration of pea using thidiazuron |
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Authors: | Massimo H M Sanago Vern I Shattuck Judith Strommer |
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Institution: | (1) Department of Horticultural Science, University of Guelph, N1G 2W1 Guelph, Ontario, Canada |
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Abstract: | A simple and rapid pea regeneration procedure was developed. An average of up to 20 shoots formed from hypocotyl explants of cvs. Sugar Ann and Patriot cultured on Murashige and Skoog basal medium supplemented with 0.5 or 1.0 M thidiazuron (N-phenyl-N -1,2,3-thiadiazol-5 -ylurea). Hypocotyls of Puget and Sugar Daddy did not respond. Regenerated shoots rooted rapidly when cultured on Murashige and Skoog basal medium containing either 2.0 M -naphthaleneacetic acid or 1.0–2.0 M indole-3-butyric acid. Seeds were harvested from regenerated plants after only 9–11 weeks.Abbreviations BAP
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxy acetic acid
- GA3
gibberellic acid
- IBA
indole-3-butyric acid
- MS
Murashige and Skoog (1962) medium
- NAA
-naphthaleneacetic acid
- TDZ
thidiazuron (N-phenyl-N -1,2,3-thiadiazol-5 -ylurea) |
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Keywords: | organogenesis Pisum sativum regeneration tissue culture |
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