Fetal cells in maternal blood: recovery by charge flow separation |
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| Authors: | S S Wachtel David Sammons Michael Manley Gwendolyn Wachtel Garland Twitty Joseph Utermohlen Owen P Phillips Lee P Shulman Douglas J Taron U R Müller Peter Koeppen Teresa M Ruffalo Karen Addis Richard Porreco Joyce Murata-Collins Natalie B Parker Loris McGavran |
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| Institution: | (1) Department of Obstetrics and Gynecology, University of Tennessee, Memphis TN 38103, USA, US;(2) BioSeparations Inc., Tucson AZ 85719, USA, US;(3) Vysis Inc., Downers Grove IL 60515, USA, US;(4) Copperstate Ob-Gyn, Tucson AZ 85712, USA, US;(5) Rocky Mountain Perinatal Associates, Denver CO 80218, USA, US;(6) The Children’s Hospital and The University of Colorado School of Medicine, Denver CO 80218, USA, US |
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| Abstract: | Fetal blood cells can be recovered from the maternal circulation by charge flow separation (CFS), a method that obviates the
risks associated with amniocentesis and chorionic villus sampling. By CFS, we processed blood samples from 13 women carrying
male fetuses, 2 carrying fetuses with trisomy 21, and 1 who had delivered a stillborn infant with trisomy 18. On average more
than 2000 fetal nucleated red blood cells were recovered per 20-ml sample of maternal blood. Recovery of fetal cells was confirmed
by fluorescence in situ hybridization with probes for chromosomes Y, 18 and 21. After culturing of CFS-processed cells, amplification
by the polymerase chain reaction revealed Y-chromosomal DNA in clones from four of six women bearing male fetuses, but not
in clones from three women bearing female fetuses.
Received: 8 January 1996 / Revised: 22 March 1996 |
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