Effects of flow on LOX-1 and oxidized low-density lipoprotein interactions in brain endothelial cell cultures |
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| Affiliation: | 1. Department of Biomedical Engineering, Columbia University, New York, NY, USA;2. Department of Radiology, Columbia University, New York, NY, USA;1. Department of Radiology, Thomas Jefferson University, 132 South 10th Street, Philadelphia, PA 19107-5244;2. Department of Radiology, The University of Texas Health Science Center at San Antonio, San Antonio, TX;3. Philips Healthcare, Cleveland, OH;1. Department of Neurosciences, Institute of Translational Neurosciences, El Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico;2. Social Sciences Department, El Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico;1. Department of Clinical Experimental Research, Glostrup Research Institute, Rigshospitalet Glostrup, Nordre Ringvej 69, 2600 Glostrup, Denmark;2. Division of Experimental Vascular Research, Department of Clinical Sciences, Lund University, Sölvegatan 17 BMC A13, 221 84 Lund, Sweden;3. Department of Chemistry, Texas A&M University, College Station, Texas 77843;4. Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843 |
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| Abstract: | Fluid shear stress and uptake of oxidized low-density lipoprotein (ox-LDL) into the vessel wall both contribute to atherosclerosis, but the relationship between shear stress and ox-LDL uptake is unclear. We examined the effects of flow, induced by orbital rotation of bEnd.3 brain endothelial cell cultures for 1 wk, on ox-LDL receptor (LOX-1) protein expression, ox-LDL uptake and ox-LDL toxicity. Orbitally rotated cultures showed no changes in LOX-1 protein expression, ox-LDL uptake or ox-LDL toxicity, compared to stationary cultures. Flow alone does not modify ox-LDL/LOX-1 signaling in bEnd.3 brain endothelial cells in vitro, suggesting that susceptibility of atheroprone vascular sites to lipid accumulation is not due solely to effects of altered flow on endothelium. |
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| Keywords: | Fluid shear stress Oxidized low-density lipoprotein (ox-LDL) Atherosclerosis Brain endothelial cell Ox-LDL receptor (LOX-1) |
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