PHA synthase engineering toward superbiocatalysts for custom-made biopolymers |
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Authors: | Christopher T Nomura Seiichi Taguchi |
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Institution: | (1) Department of Chemistry, State University of New York–College of Environmental Science and Forestry, 121 Jahn Laboratory, Syracuse, NY 13210, USA;(2) Division of Biotechnology and Macromolecular Chemistry, The Graduate School of Engineering, N13W8, Kita-Ku, Hokkaido University, Sapporo 060-8628, Japan |
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Abstract: | Poly-3-hydroxyalkanoates P(3HA)s] are biologically produced polyesters that have attracted much attention as biodegradable
polymers that can be produced from biorenewable resources. These polymers have many attractive properties for use as bulk
commodity plastics, fishing lines, and medical uses that are dependent on the repeating unit structures. Despite the readily
apparent benefits of using P(3HA)s as replacements for petrochemical-derived plastics, the use and distribution of P(3HA)s
have been limited by their cost of production. This problem is currently being addressed by the engineering of enzymes involved
in the production of P(3HA)s. Polyhydroxyalkanoate (PHA) synthase (PhaC) enzymes, which catalyze the polymerization of 3-hydroxyacyl-CoA
monomers to P(3HA)s, were subjected to various forms of protein engineering to improve the enzyme activity or substrate specificity.
This review covers the recent history of PHA synthase engineering and also summarizes studies that have utilized engineered
PHA synthases. |
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Keywords: | Polyhydroxyalkanoates PHA synthase Genetic engineering In vitro evolution Escherichia coli Ralstonia eutropha Arabidopsis thaliana PHA monomer-supplying enzymes |
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