Efficient Method for Analysis of QTL Using F1 Progenies in an Outcrossing Species |
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Authors: | Takeshi Hayashi Takashi Awata |
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Institution: | Laboratory of Animal Genome, National Institute of Agrobiological Sciences, Tsukuba-shi, Ibaraki 305-0901, Japan. hayatk@affrc.go.jp |
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Abstract: | In the present paper, we proposed a statistical procedure based on composite interval mapping for accurate analysis of quantitative trait loci (QTL) for individuals sampled from an outcrossing population with two-generation families consisting of the sampled individuals and F1 progenies obtained by crossing them as parental individuals. In the proposed procedure, haplotypes of markers of parental individuals were reconstructed based on the genotypes of F1 progenies and QTL analyses with composite interval mapping were conducted separately for each of parents as well as jointly for both parents. A least squares method was applied to the composite interval mapping, where some of markers were selected as cofactors to absorb the variation induced by QTL located elsewhere in the genome. The procedure was evaluated for the efficiency in detecting QTL and the precision of estimates of locations and effects of QTL using simulations. It was shown that QTL with interaction between paternal and maternal alleles was effectively detected by joint analysis of both parents, while a QTL segregating only in one parent, closely linked to a QTL segregating only in the other parent, was successfully detected by analyzing separately each of the parents with inclusion of markers of both parents. The proposed procedure can provide detailed genetic information useful for marker assisted breeding in an outcrossing species such as forest trees. |
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Keywords: | composite interval mapping haplotype least squares method outcrossing species QTL analysis |
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