Cross-reactivity of bacteriocins from lactic acid bacteria and lantibiotics in a nisin bioassay and ELISA |
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Authors: | MB Falahee MR Adams |
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Institution: | School of Biological Sciences, University of Surrey, Guildford, UK. |
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Abstract: | A number of bacteriocins from lactic acid bacteria and lantibiotics were tested for cross-reactivity in a nisin ELISA and bioassay. The bacteriocins showed no cross-reactivity, reflecting their structural dissimilarity from nisin. The lantibiotic subtilin which shares many common structural features with nisin, showed a high cross-reactivity in both the ELISA and the bioassay suggesting possible modifications to nisin to enhance its activity. Gallidermin did not cross react in the ELISA but did produce a zone of inhibition in the less specific bioassay. Other lantibiotics tested did not react in either assay. |
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