裂褶菌培养基形态学观察和DNA序列分析

目的通过观察裂褶菌在5种培养基上的生长状态、扫描电镜及DNA序列分析,了解该菌形态学及分子生物学等方面的特征。方法菌落转种于沙氏培养基(SDA),麦芽浸膏琼脂(MEA),马铃薯葡萄糖琼脂(PDA),玉米粉琼脂(CMA)和察氏琼脂(CZA)平皿培养基,27℃和37℃培养2周,观察菌落生长情况,进行扫描电镜检测及DNA序列分析。结果菌落在SDA,MEA和PDA上生长状态较好,呈蓬松白色羊毛状;尿素酶试验阳性,放线菌酮耐受试验阴性。光镜下见分支分隔菌丝、侧生的钉状突起及类水母体变异子实体。扫描电镜见菌丝分隔处闭锁联合、侧生钉状突起和泪滴状球形分泌物。经26S rDNAD1/D2区序列分析证实该菌株为裂褶菌。结论裂褶菌只有丝状型一种菌落形态;分支分隔菌丝及分隔处闭锁联合,侧生钉状突起和泪滴状球形分泌为其形态学特征;孢子由类水母状子实体产生。

Morphology and sequence DNA analysis of Schizophyllum commune

Objective To observe morphological characteristics of Schizophyllum commune on five kind of culture media.Methods Schizophyllum commune was cultured on Sabouraud＇s Agar（SDA）,Malt Extract Agar（MEA）,Patato Glucose Agar（PDA）,Corn Meal Agar（CMA） and Czapek＇s Agar（CZA） respectively at 37℃ and 27℃ for two weeks.Scanning electron microscopy and DNA sequence analysis were performed after one-week culture.Results White and woolly colonies growed better on SDA,MEA and PDA with positive result of urease test and regative result of cycloheximide tolerance test.Septatus mycelium with branches were found under light microscope,and branched hyphae with clamp connections,short and curved lateral pegs and waterdrop-shape excretion were detected under scanning electron microscope.Schizophyllum commune was identified by sequence analysis of 26S rDNA D1/D2.Conclusions Schizophyllum commune had an unique colonial morphology of hyphomycete with characteristic clamp connections,short lateral spicules and waterdrop-like excretion.