Separation of the cytoplasmic and outer membrane of Pseudomonas aeruginosa PAQ. |
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Authors: | B R Booth N A Curtis |
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Affiliation: | University of Bristol, Department of Bacteriology, The Medical School, University Walk, Bristol, BS8 1TD UK;Glaxo Research Ltd., Greenford, Middlesex, UB6 0HE UK |
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Abstract: | A method is described for the isolation of the cytoplasmic and outer membranes of PAO.1. The cytoplasmic membrane exhibits nicotinamide adenine dinucleotide oxidoreductase, lactate dehydrogenase DD-carboxypeptidase and succinate dehydrogenase activities. The outer membrane is rich in 2-keto-3-deoxyoctonate and exhibits phospholipase A and DD-carboxypeptidase activity. At least 25 protein species have been detected in the cytoplasmic membrane by polyacrylamide gel electrophoresis. Using the same technique, the outer membrane contains only five protein species of molecular weights, 56,000, 53,000, 38,000, 21,000 and 16,000. |
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