The determination of the optimal conditions for the production of a number of pathogenicity factors in Vibrio cholerae

Conditions for the cultivation of V. cholerae of different sero- and biovars on tryptone medium, ensuring the maximum production of cholera toxin (CT), dermonecrotic factor (DNF), hemorrhagic factor (HF) and new cholera toxin (NCT) have been determined. The lack of coincidence in the optimum conditions ensuring the maximum production of CT, DNF and HF has been established, which may be indicative of different nature of these toxic substances. NCT, produced by vct- strains, is similar to CN in biological activity as determined in the skin permeability test and in the conditions of accumulation in tryptone medium.