Quantitative characterization of cellular differentiation of Streptomyces ambofaciensin submerged culture by image analysis |
| |
| Authors: | JF Drouin L Louvel B Vanhoutte H Vivier MN Pons P Germain |
| |
| Institution: | (1) Laboratoire des Sciences du Génie Chimique CNRS-ENSIC-INPL, 1, rue Grandville, BP 451, F-54001 Nancy cedex, France;(2) Laboratoire de Microbiologie Industrielle ENSAIA-INPL, 2 avenue de la Forêt de Haye, BP 172, F-54505 Vandoeuvre-les-Nancy cedex, France;(3) Rhône-Poulenc Rorer SA, 13 quai Jules Guesde, BP 14, F-94400 Vitry-sur-Seine, France;(4) CRITT, Rhône-Poulenc, F-69000 Décines, France |
| |
| Abstract: | Image analysis methods were developed for light and epifluorescence microscopy of Streptomyces ambofaciens undergoing differentiation in submerged culture. Grey level images were obtained with an integration controlled CCD camera and allowed three parameters to be measured: occurence of empty zones in mycelium, number of septations, mycelium thickness. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
